Figure 3. Effect of naringenin (Nar) on senescence of mouse neural stem cells (mNSCs). (A) p16ink4a mRNA expression was measured by qRT-PCR in 23M-NSCs with and without treatment of 6.8 μg/mL Nar for 48 h. (B) Cell cycle phase distributions of 23M-NSCs treated with Nar for 48 h and control cells. (C) The relative telomere length of 23M-NSCs increased significantly with Nar treatment. (D) Immunofluorescence Ki67 staining of mNSCs treated with Nar for 48 h, with DAPI nuclear labeling. (E) Quantification of (D). (F) Representative fields of MAP2 and GFAP immunofluorescence staining of cultured mNSCs after control and Nar treatment. (G) Quantification of (F). Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.0001 compared with untreated cells.