Research Paper Volume 12, Issue 24 pp 25020—25034

TRIB3 facilitates glioblastoma progression via restraining autophagy

Figure 3. Knockdown of TRIB3 impairs the proliferation and migration of GBM cells. (A) The effect of TRIB3 knockdown on U251 and U87 cell proliferation was detected by CCK-8 assay at the indicated times. (B) The colony formation of U251 and U87 cells treated with si-NC and si-TRIB3. (C) Migration ability, and invasion ability were determined after the downregulation of TRIB3 in glioma cell lines using a migration assay and a Transwell assay. (D) The migration potential of U251 and U87 cells treated with si-NC and si-TRIB3 was evaluated by the wound healing test. *p<0.05, **p< 0.01.