Figure 2. LINC01235 affects gastric cancer metastasis in vitro and vivo. (A) Wound-healing percentages at 24 h were largely inhibited in LINC01235-depleted HGC-27 and SGC-7901 cells, compared with control. In (B) migration and (C) Matrigel invasion assays, the number of migrated cells significantly decreased in LINC01235-silenced HGC-27 and SGC-7901 cells, compared with the control. (D) GSEA shows that LINC01235 may have a vital function in EMT. (E) RT-qPCR analyses of effects of LINC01235 depletion on expression of EMT-related genes in HGC-27 cells. (F) Immunofluorescence assay shows the expression of EMT in control/LINC01235-silenced cells. Green indicates E-cadherin and red indicates N-cadherin. (G) Representative tumor nodules in lungs of nude mice that were intravenously injected with control and LINC01235-silenced HGC-27 cells. Results are expressed as means ± SD. ** and ***: P < 0.01 and 0.001, respectively.