Research Paper Volume 12, Issue 24 pp 25060—25075

LINC01235-TWIST2 feedback loop facilitates epithelial–mesenchymal transition in gastric cancer by inhibiting THBS2

Figure 4. LINC01235 depletion downregulates TWIST2 expression. (A) Predicted correlation between LINC01235 and TWIST2 in the TCGA gastric cancer data set. (B) RT-qPCR shows correlations between LINC01235 and TWIST2 expression in 48 additional GC tissues. (C) RT-qPCR shows TWIST2 expression in control- and siLINC01235-HGC-27 cells. (D) Western blot shows effects of ectopic TWIST2 on expression of EMT-related genes in control- and siLINC01235-HGC-27 cells. (E and F) Wound-healing percentages in control, siLINC01235, and siLINC01235+TWIST2 groups. (G and H) Transwell assay of HGC-27 and SGC-7901 cells shows effects of LINC01235 and TWIST2 on migration and invasion. Results are expressed as means ± SD. **: P < 0.01.