Research Paper Volume 12, Issue 24 pp 25060—25075

LINC01235-TWIST2 feedback loop facilitates epithelial–mesenchymal transition in gastric cancer by inhibiting THBS2

Figure 5. THBS2 is the apparent target gene of the LINC01235–TWIST2 axis. (A) The protein levels of TWIST2 and THBS2 were diminished by ectopic transfection of cells with strengthened LINC01235. (B) Western blot analysis of expression of E-cadherin, N-cadherin, and vimentin proteins in HGC-27 and SGC-7901 cells transfected with GFP-vector, GFP-TWIST2, and GFP-TWIST2/THBS2. (C) Low and high expression of TWIST2 and THBS2 in GC specimens. (D) TWIST2 and THBS2 expression by immunofluorescence staining in cancer and adjacent normal tissues. Green indicatesTWIST2, and red indicates THBS2. (E and F) Correlation between TWIST2 and THBS2 IHC scores and immunofluorescence scores. Kaplan–Meier analysis showed that (G) high expression of TWIST2 and (H) low expression of THBS2 predicted poor prognosis for GC patients.