Research Paper Volume 12, Issue 24 pp 25090—25100

miR-100-3p inhibits the adipogenic differentiation of hMSCs by targeting PIK3R1 via the PI3K/AKT signaling pathway

Figure 4. miR-100-3p specifically binds to the PIK3R1 3′-UTR region. (A) Illustration of the putative site of miR-100-3p binding within the 3’-UTR of PIK3R1, with nucleotides that were mutated for MUT reporter preparation highlighted in red. (B) Luciferase activity assay results. (C, D) The impact of miR-100-3p overexpression on PIK3R1 expression in the context of hMSC adipogenesis was measured via qRT-PCR and Western blotting. Data are means ± SD (n=3). **P<0.01 vs. negative controls, respectively. Note: NC: negative control; miR-100-3p: miR-100-3p overexpression.