Figure 6. miR-100-3p controls hMSC adipogenesis via the PI3K/AKT signaling pathway. hMSCs overexpressing miR-100-3p were treated with a PI3K/AKT signaling pathway agonist. (A) Protein expression in these cells was assessed. (B) Lipid droplets were detected in these cells via Oil Red O staining; scale bar, 20 μm. (C) Oil Red O staining intensity differed significantly among study groups. Data were collected on day 7 post-adipogenic induction. Data are means ± SD (X ± SD, n=3). **P<0.01 vs negative control, ##P<0.01 vs miR-100-3p overexpression. Note: NC: negative control; miR-100-3p: miR-100-3p overexpression, 740Y-P: PI3K/AKT agonist 740Y-P.