Research Paper Volume 12, Issue 24 pp 25138—25152

Bone marrow mesenchymal stem cell-derived exosomes prevent osteoarthritis by regulating synovial macrophage polarization

Figure 7. Macrophages treated with BMSC exosomes maintain chondrogenic characteristics and inhibit hypertrophy of chondrocytes. (A) and (B) Detection of the expression of collagen II by immunofluorescence in chondrocytes and statistical results. Scale bar: 100 μm. (C) and (D) Detection of the expression of collagen X by immunofluorescence in chondrocytes and statistical results. Scale bar: 100 μm. (EI) Detection of the expression of collagen II, collagen X, SOX9 and Runx2 in chondrocytes by western blotting and statistical results. (J) Image of toluidine blue staining of chondrocytes. Scale bar: 200 μm. *p < 0.05 compared to the Control group; # p < 0.05 compared to the LPS group.