Research Paper Volume 12, Issue 24 pp 25189—25206

Histone methyltransferase SETD2 inhibits tumor growth via suppressing CXCL1-mediated activation of cell cycle in lung adenocarcinoma

Figure 4. Clinical relevance of CXCL1 in human LUAD. (A) CXCL1 was up-regulated upon SETD2 depletion as indicated in volcano plot. (B) Real-time qPCR analysis of CXCL1 expression level upon SETD2 overexpression or depletion in H1650 and PC-9 cells. (C) Real-time qPCR analysis of CXCL1 expression in HBE cells and human lung cancer cell lines A549, H1975, H1299, H1650 and PC-9. (D) Scatter plot of CXCL1 expression levels in LUAD tumors and adjacent normal epithelial tissues (left panel). The association (by Pearson’s) between SETD2 and CXCL1 expressions in patients (right panel). (E) Kaplan-Meier plot of overall survival based on the CXCL1 expression index in patients (P values by log-rank test). *P<0.05. Two-tailed Student’s t test. Pearson’s correlation test was used to analysis the correlation between CXCL1 and SETD2.