Figure 1. Pol β-deficient mouse ovarian function and impaired BER lead to ovarian aging. (A) The modeling strategy of pol β+/- mice. (B) Relative Pol β gene expression levels in Pol β-deficient mice. We observed significant Pol β deficiency in heterozygous Pol β+/- mice compared with that in wt mice (mice aged 6-8 weeks, n stands for the number of mice, ***P<0.001, Student’s t test). (C) To confirm that the Pol β fragment was deleted correctly, total RNA was extracted and Pol β cDNA was amplified via PCR.