Research Paper Volume 12, Issue 24 pp 25207—25228

Impairment of Pol β-related DNA base-excision repair leads to ovarian aging in mice

Figure 1. Pol β-deficient mouse ovarian function and impaired BER lead to ovarian aging. (N) Pol β+/- (6-8 weeks) and wt (6-8 weeks) oocytes were immunofluorescent for AC3. Pol β+/- showed a significant increase in AC3 fluorescence intensity compared to wt (n stands for the number of oocytes, **P<0.01, Student’s t test). (O) Pol β+/- (6-8 weeks) ovarian tissue showed a higher AC3 fluorescence intensity (n stands for the number of oocytes, *P < 0.05, Student’s t test). The pink arrow indicates normal oocytes; the white arrow indicates apoptotic oocytes. (P) 5-FU treatment results in ovarian tissue with greater AC3 fluorescence intensity (mice aged 6-8 weeks, n stands for the number of oocytes, ***P <0.001, Student’s t test). The pink arrow indicates normal oocytes; the white arrow indicates apoptotic oocytes (mice injected with 5-FU for 2 weeks under the safe dose of 20 mg/kg every day, Student’s t test). All scatter graphs and bar graphs show the means ± SD.