Research Paper Volume 12, Issue 24 pp 25229—25255

Exposure to ozone impacted Th1/Th2 imbalance of CD4+ T cells and apoptosis of ASMCs underlying asthmatic progression by activating lncRNA PVT1-miR-15a-5p/miR-29c-3p signaling

Figure 8. PI3K/AKT/mTOR signaling was implicated in the contribution of PVT1/miR-15a-5p axis to Th1/Th2 balance in CD4+ T cells. (A) PVT1 expression was determined after transfection of pcDNA3.1-PVT1 and si-PVT1, and miR-15a-5p level was compared in CD4+ T cells transfected by miR-15a-5p mimic and inhibitor. *: P<0.05 when compared with miR-NC group. (B) The p-PI3K, PI3K, p-AKT, AKT, p-mTOR and mTOR expressions in CD4+ T cells were figured out among NC, pcDNA3.1-PVT1, si-PVT1, miR-15a-5p mimic and miR-15a-5p inhibitor groups. *: P<0.05 when compared with NC group. (C) PVT1 and miR-15a-5p expressions were obtained from CD4+ T managed by IGF-1 and LY294002. *: P<0.05 when compared with NC group. (D) The levels of cytokines relevant to Th1/Th2 imbalance were determined within CD4+ T cells among miR-NC, miR-15a-5p mimic and miR-15a-5p mimic+IGF-1 groups. *: P<0.05 when compared with miR-NC group, #: P<0.05 when compared with miR-15a-5p mimic group.