Figure 3. qRT-PCR, Western blot and IHC measurements of SRD5A3 in HCC and peri-HCC tissues. (A) The mRNA expression of SRD5A3 in 36 pairs of HCC and peri-HCC tissues was measured by qRT-PCR analysis. The SRD5A3 mRNA levels of peri-HCC tissues were normalized as ‘1’. (B, C) The protein level of SRD5A3 in 7 pairs of HCC and peri-HCC tissues was analyzed by Western blot. (D) The location of SRD5A3 protein was detected by IHC staining. T, HCC tumor tissue; N, peri-HCC tumor tissue.