Research Paper Volume 12, Issue 24 pp 25395—25411

Steroid 5 alpha-reductase 3 (SRD5A3) promotes tumor growth and predicts poor survival of human hepatocellular carcinoma (HCC)

Figure 7. SRD5A3 inhibition suppresses cell proliferation of HCC in vitro. SMMC-7721 and QGY-7703 cell lines were infected with sh-SRD5A3 or sh-NC lentivirus. The knockdown efficiency of SRD5A3 was confirmed by (AC) Western blot and (D, E) qRT-PCR analyses. (FH) Colony formation assays were performed and analyzed. (I, J) CCK-8 assays were conducted to measure cell viability (two-way ANOVA analysis). NC, negative control. *, P value < 0.05.