Figure 2. Differentially methylated regions and expression. (A, B) The DMR located in promotors and expression of SPACA1 and MEI1, respectively. (C) The differential transcription levels and methylation levels of TNNT3 in muscles and testes. Bar plot shows the reads coverage of RNA-seq data from muscles (top), line chart shows the methylation levels of TNNT3 in both tissues (middle), the scatter plot shows the methylation levels and distribution of DMCpGs in TNNT3, and box plot shows the transcription levels of TNNT3 in both tissues (bottom). (D) Methylation level in DMR of TCEA3’s upstream was positively correlated with expression. (E) Prediction of transcription factor binding sites for conserved sequences.