Figure 2. OVA-treated DCs stimulated T-cells to differentiate into Th1, Th2 and Treg cell lines. (A, B). Naïve CD4+ T cells were isolated using MACS from mouse systemic lymph nodes and were co-cultured for 72 h with DCs pretreated with different doses of OVA. Data indicate the mean±SD, n = 4. Percentage T cells is tested by flow cytometry. *p<0.05 represents the differences between the treatment group and the control group. *(P<0.05) represents the differences among the five dose groups.