Figure 2. SPC inhibited necroptosis and the up-regulation of RIPK3, MLKL proteins induced by MIRI. (A) Analysis of IR injury induced necroptosis via Evans blue dye uptake in the hearts. (B)The percent area of EBD positive myocardium is shown. n=3/group. (C) Immunoblotting was used to examine the expression levels of p-RIPK1, RIPK1, p-RIPK3, RIPK3, p-MLKL and MLKL proteins. Representative protein images and quantitative analysis were shown. n = 3 /group. The columns and errors bars represent means ± SD. * P < 0.05 vs. SHAM group; # P < 0.05 vs. IR group.