Research Paper Volume 13, Issue 1 pp 340—350

LINC00052 ameliorates acute kidney injury by sponging miR-532-3p and activating the Wnt signaling pathway

Figure 3. miR-532-3p is a target of LINC00052. (A) A schematic of the interaction between LINC00052 and miR-532-3p. (B) The luciferase reporter constructs containing the wild type (WT-LINC00052) or mutant LINC00052 (MUT-LINC00052). (C) The relationship between LINC00052 and miR-532-3p was assessed using a dual luciferase reporter assay. WT-LINC0005 or MUT-LINC00052 were co-transfected into NRK-52E cells with miR-532-3p mimics or their corresponding negative controls. (D) The interaction between LINC00052 and miR-532-3p was assessed using a RIP assay. (E) RNA pull-down assays detected a direct interaction between LINC00052 and miR-532-3p. Three independent experiments were performed. Error bars represent the mean ± SD of triplicate experiments (at least). *p < 0.05.