Research Paper Volume 12, Issue 24 pp 25505—25527

Sesn2 attenuates the damage of endothelial progenitor cells induced by angiotensin II through regulating the Keap1/Nrf2 signal pathway

Figure 3. Knockdown of Nrf2 could reverse the protective effect of Sesn2 in EPCs treated with Ang-II. EPCs were divided into treatment groups including for a control, Ang-II, Ang-II + Lenti-Sesn2, and Ang-II+ Lenti-Sesn2 + si-Nrf2 treatment groups. (A) The levels of Nrf2 mRNA were found to have decreased in EPCs transfected with siNrf2. (B) The levels of Nrf2 proteins were found to have been reduced in EPCs transfected with si-Nrf2. (C) Measures for Sesn2, Nrf2, Cleaved-caspase3, Bcl-2, Bax, and GAPDH protein expression in EPCs were determined by using Western blotting. (D) We used immunofluorescence to detect levels of caspase3 protein in ECPs. Scale bars = 2 μm. (E) We determined the migration of EPCs by using Transwell assays. Scale bars = 5 μm. All experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, versus the control. Data are represented as mean +/- SEM.