Figure 5. Sesn2 protected the EPCs through regulating the autophagic flux. EPCs were divided into treatment groups representing the control, si-Sesn2, and si-Sesn2 + CQ. (A) The levels of P62, LC-3, Cleaved-caspase3, Keap1, Nrf-2, and of GAPDH proteins in EPCs. (B) Measures of ROS production in EPCs were assessed by the use of fluorescent dye H2DCFDA. Scale bars = 5 μm. (C) Measures of the migration of EPCs were determined by using Transwell assays. Scale bars = 20 μm. All experiments were performed in triplicate. Scale bars = 5 μm. *p < 0.05, **p < 0.01, ***p < 0.001, versus the control. Data are represented as mean +/- SEM.