Figure 7. NAC facilitated the survival and migration of EPCs after silencing of Nrf2. EPCs were divided into treatment groups representative of the control, si-Nrf2 and, si-Nrf2 + NAC. (A) The levels of Cleaved-caspase3, Bax, Bcl-2, Beclin-1, LC-3, and GAPDH proteins in EPCs were determined by using Western blotting. (B) We used Hoechst 33258 staining to detect the apoptosis of EPCs. Scale bars = 5 μm. (C) Migration of EPCs was determined by the application of wound healing assays (details given in methods). All experiments were performed in triplicate. Scale bars = 20 μm. *p < 0.05, **p < 0.01, ***p < 0.001, versus the control. Data are represented as mean +/- SEM.