The roles of TRIM27 and SIX3 on lung metastasis in vivo. (A) Histology of single lung lobe from mice intravenously injected with A549 cells stably infected with blank pLVX-Puro vector, pLVX-Puro-TRIM27, or pLVX-Puro-SIX3. Scale bars: 500 μm. (B) Quantification of microscopic nodules in the lungs of each group. (C) The incidence of metastasis in mice after intravenous tail injection of each cell type is shown in the table. All experiments were repeated at least three times, and data are represented as mean ± SD. *P < 0.05, ***P < 0.001 (two-way ANOVA followed by Dunnett’s test).