Research Paper Volume 12, Issue 24 pp 25581—25598

A dual inhibitor targeting HMG-CoA reductase and histone deacetylase mitigates neurite degeneration in LRRK2-G2019S parkinsonism

Figure 1. JMF3086 synergistically mitigates neurite degeneration in primary hippocampal neurons from transgenic LRRK2-G2019S mice compared to treatment with lovastatin or SAHA. (AE) Representative images showing cultured primary hippocampal neurons (DIV 14) from (A) non-transgenic littermate control (nTg) and (BE) LRRK2-G2019S pups treated with (B) vehicle DMSO or (CE) JMF3086 at different concentrations. Neurites were stained with anti-MAP2 antibody. Scale bar, 100 μm. (F) Quantitative analysis of mean total neurite length for the neurons described in A–E, with treatment using different concentrations of lovastatin and SAHA. We analyzed 50–100 neurons for each genotype or treatment condition. Data represent mean ± SEM. *P<0.05, **P<0.01.