Research Paper Volume 12, Issue 24 pp 25581—25598

A dual inhibitor targeting HMG-CoA reductase and histone deacetylase mitigates neurite degeneration in LRRK2-G2019S parkinsonism

Figure 2. JMF3086 synergistically promotes neurite arborization in primary nigral TH-positive neurons from transgenic LRRK2-G2019S mice compared to treatment with lovastatin or SAHA. (AE) Representative images showing cultured primary nigral TH-positive neurons (DIV 14) from (A) non-transgenic littermate control (nTg) and (BE) LRRK2-G2019S pups treated with (B) vehicle DMSO or (CE) different concentrations of JMF3086. Neurites were stained with anti-TH antibody. Scale bar, 100 μm. (F) Quantitative analysis of mean total neurite length for the TH(+) neurons described in A–E, with treatment using different concentrations of lovastatin and SAHA. We analyzed 30–50 neurons for each genotype or treatment condition. Data represent mean ± SEM. *P<0.05, **P<0.01.