Research Paper Volume 12, Issue 24 pp 25643—25657

Neurofilament degradation is involved in laparotomy-induced cognitive dysfunction in aged rats

Figure 5. TSA pretreatment reduces surgery-induced upregulation of NFs in the hippocampus of aged rats. (A) iTRAQ analyses for the NFs proteins at 6 h post-surgery in proteomics. (B) Immunofluorescence analysis and (C) semi-quantification of the NFs reveals it increased in the hippocampal CA1 region at 6 h after surgery, which was significantly inhibited by TSA pretreatment (cell nuclei, blue; NEFH, purple; NEFM, green; NEFL, orange. Magnification 400×, Scale bar = 50 μm). (D) Representative western blotting images and statistical analysis of NEFH, NEFM and NEFL are shown. Data are given as means {plus minus} SEM, n = 4. *P < 0.05 and **P < 0.01 vs. the control group; #P < 0.05 vs. the surgery group.