Research Paper Volume 12, Issue 24 pp 25658—25672

Up-regulation of circRNA-0003528 promotes mycobacterium tuberculosis associated macrophage polarization via down-regulating miR-224-5p, miR-324-5p and miR-488-5p and up-regulating CTLA4

Figure 3. Hsa_circ_0003528 functioned as a sponge of miR-324-5p, miR-224-5p and miR-488-5p, while CTLA4 mRNA was targeted by miR-224-5p, miR-324-5p and miR-488-5p (*P value < 0.05 vs. control group). (A) Computational analysis and luciferase assay of the molecular relationship between hsa_circ_0003528 and miR-324-5p; (B) Computational analysis and luciferase assay of the molecular relationship between hsa_circ_0003528 and miR-224-5p; (C) Computational analysis and luciferase assay of the molecular relationship between hsa_circ_0003528 and miR-488-5p; (D) Computational analysis and luciferase assay of the molecular relationship between CLTA4 mRNA and miR-324-5p; (E) Computational analysis and luciferase assay of the molecular relationship between CLTA4 mRNA and miR-224-5p; (F) Computational analysis and luciferase assay of the molecular relationship between CLTA4 mRNA and miR-488-5p.