Research Paper Volume 12, Issue 24 pp 25673—25683

Loss of Atg7 causes chaotic nucleosome assembly of mouse bone marrow CD11b+Ly6G- myeloid cells

Figure 4. Atg7 deletion accelerates the aging of CD11b+Ly6G- myeloid cells. (A) Flow cytometric analysis of ROS levels of CD11b+Ly6G- cells with fluorescent DCFH-DA. Left, histogram for flow cytometric assessment of CD11b+Ly6G- cells; right, geometric mean fluorescence intensity (MFI) of DCFH-DA in CD11b+Ly6G- cells of wild-type mice and atg7-deleted mice. (B) Flow cytometric analysis of mitochondrial mass levels of CD11b+Ly6G- cells with florescent MitoTracker Deep Red. Left, histogram for the flow cytometric assessment of CD11b+Ly6G- cells; right, geometric MFI of MitoTracker Deep Red in CD11b+Ly6G- cells of wild-type and atg7-deleted mice. (C) Gene expression heatmap of cell death negative regulators. (D) Analysis of apoptosis in CD11b+Ly6G- cells of wild-type mice and Atg7-deleted mice by Annexin V and PI double staining. Left, representative flow cytometric measurement; right, statistical results from cytometric analysis. Early apoptosis, Annexin V+PI-; late apoptosis, Annexin V+PI+ (right)