Figure 4. Cardamonin treatment induces apoptosis and inhibits the mTOR pathway in ovarian cancer cells. (A) Representative images of apoptosis detected with Annexin V-FITC and PI staining in SKOV3 cells and PDC. Cells were treated with different concentrations of cardamonin for 48 h. Percentages in the bottom right and top right quadrants represent early and late apoptosis, respectively. (B) Western blot showing change of protein expressions of Mcl-1, Bax, Bcl-2, cytochrome C, caspase-3 and cleaved caspase-3. (C) Western blot showing chang of protein expression of mTOR, p-mTOR, p-PRAS40, Ragc, and p-Raptor. β-actin was included as a loading control.