Figure 5. NF-κB mediates the anti-proliferative effect of cardamonin in ovarian cancer cells. (A) SKOV3 cells and PDC were cultured with 15 μM cardamonin or 0.1% DMSO for 24 h and intracellular distribution of NF-κB subsequently analyzed via immunofluorescence. (B) Western blot showing change of protein expressions of NF-κb, p-NF-κB, IKKα, IKKβ, p-IKKα/β, IKBα and p-IKBα in cells after treatment with the indicated concentrations of cardamonin. β-actin was included as a loading control.