Research Paper Volume 12, Issue 24 pp 25730—25743

Cardamonin induces G2/M phase arrest and apoptosis through inhibition of NF-κB and mTOR pathways in ovarian cancer

Figure 6. Cardamonin induces cell cycle arrest in SKOV3 and PDC cells through NF-κB and mTOR pathways. (A) Representative results of flow cytometry analysis of SKOV3 cells and PDC treated with cardamonin (15 μM) or PS-341 (50 nM). Graphs depicting changes in the percentage of cells at each phase of the cell cycle. (B) Representative results of flow cytometry analysis of SKOV3 and PDC treated with cardamonin (15 μM) or rapamycin (20 nM). Graphs depicting changes in the percentage of cells at each phase of the cell cycle. (C) Western blots showing the effects of cardamonin (15 μM) and PS-341 (50 nM) on expression of cyclin D1, cyclin A, cyclin B1, p-cdc2, Myt1 and p-Histone H3. (D) Western blots showing the effects of cardamonin (15 μM) and rapamycin (20 nM) on expression of cyclin D1, cyclin A, cyclin B1, p-cdc2, Myt1 and p-Histone H3. Data represent average results from three independent experiments; SD signifies standard deviation (n=3), ##P<0.01 indicates significance.