Research Paper Volume 12, Issue 24 pp 25744—25766

Alisol B 23-acetate activates ABCG5/G8 in the jejunum via the LXRα/ACAT2 pathway to relieve atherosclerosis in ovariectomized ApoE-/- mice

Figure 2. AB23A improves the plasma lipid fraction of ovariectomized ApoE-/- mice. (A, B) Kits were used to measure the levels of total cholesterol (TC), total triglycerides (TG), high-density lipoprotein (HDL-c) and low-density lipoprotein in plasma (LDL-c). The remaining plasma lipids were extracted and detected by HPLC-Q-TOF/MS, and statistical analysis was performed according to different mathematical models. (C) The volcano plot shows the difference in the lipid metabolites between the AB23A-treated group and the control group. (D) PCA score chart showing the differences in the plasma samples from the AB23A-treated group (green) and the model control group (red). PC1=72.4%, PC2=11.0%. (E) The OPLS-DA score chart shows the AB23A-treated group (Orange) and the control group (Red). The adaptability and predictive ability are expressed as follows: T score1=70.9%, Orthogonal T score1=7.5%. (F) The heat map shows that after screening, AB23A can significantly affect the different classes of plasma lipids. (GI) The box plots show the relative lipid content of each category. The data are expressed as the mean ± SEM (n=6/group).