Alisol B 23-acetate activates ABCG5/G8 in the jejunum via the LXRα/ACAT2 pathway to relieve atherosclerosis in ovariectomized ApoE^{-/-} mice

Figure 3.AB23A maintains the structural stability of the jejunum in ovariectomized ApoE^{-/-} mice by improving the jejunal lipid mass spectrum. Ovaries were removed from 8-week-old female ApoE^{-/-} mice, and the mice were fed a high-fat diet with saline or AB23A (2.55 mg/kg) daily for 12 weeks. The proximal jejunum lipids were extracted and detected by HPLC-Q-TOF/MS, and statistical analysis was performed according to different mathematical models. (A, B) Representative image of oil red O staining of the proximal jejunum. Original magnification: 40×. (C, D) Representative HE staining of ovariectomized ApoE^{-/-} mice with proximal jejunal lesions. Original magnification: 20×. (E) Quantification of the lipid area in the villi (n=5/group). p-values represent comparisons with the control group. The data are expressed as the mean ± SEM (n=5/group). (F) The volcano plot shows the difference in the lipid metabolites between the AB23A-treated group and the control group. (G) The PCA score chart shows the differences in the plasma samples from the AB23A-treated group (Orange) and the model control group (Red). PC1=84.7%, PC2=11.4%. (H) The OPLS-DA score chart shows the AB23A-treated group (Orange) and the control group (Red). The adaptability and predictive ability are expressed as follows: T score1=74.3%, Orthogonal T score1=19.7%. (I) The heat map shows that after screening, AB23A can significantly affect the different classes of plasma lipids. (J–M) The box plots show the relative lipid content of each category. The data are expressed as the mean ± SEM (n=5/group).