Figure 5. AB23A reduces lipid accumulation in Caco-2 cells cultured under high-fat conditions. Caco-2 cells cultured under high-fat conditions were exposed to different concentrations of AB23A (0, 20, 40, or 80 μM) for 24 h. (A) Representative image of Oil Red O staining of Caco-2 cells. Original magnification: 200×. (B) Quantification of the intracellular lipid droplet area (n=5/group). (C–E) Kits were used to measure the levels of total cholesterol, total triglycerides, free cholesterol, and cholesterol esters in Caco-2 cells. All the results were obtained from three independent experiments. p-values represent comparisons with controls. The values represent the mean ± SEM (n=6/group).