Figure 6. AB23A promotes the expression of ABCG5 and ABCG8 by activating LXRα. Caco-2 cells cultured under high-fat conditions were exposed to different concentrations of AB23A (0, 20, 40, or 80 μM) for 24 h. (A–C) The protein and mRNA expression of ACAT2, ABCG5 and ABCG8 in Caco-2 cells from different groups (n=3/group). (D–F) The LXRα inhibitor GSK2033 was added with 80 μM AB23A for 24 h. RT-qPCR and Western blot analyses were performed to evaluate the mRNA and protein levels of LXRα, ACAT2, ABCG5, and ABCG8 (n=3/group). (G) Representative image of Oil Red O staining of Caco-2 cells. Original magnification: 200×. (H) Quantification of the intracellular lipid droplet area (n=5/group). The data are expressed as the mean ± SEM, and the results were obtained from three independent experiments. *P <0.05 compared to the control group; #P <0.05 vs the AB23A only group.