Research Paper Volume 12, Issue 24 pp 25744—25766

Alisol B 23-acetate activates ABCG5/G8 in the jejunum via the LXRα/ACAT2 pathway to relieve atherosclerosis in ovariectomized ApoE-/- mice

Figure 7. The LXRα-ACAT2 pathway is involved in the upregulation of ABCG5 and ABCG8 by AB23A. In Caco-2 cells cultured under high-fat conditions, 80 μM AB23A and ACAT2 inhibitors were simultaneously administered and incubated for 24 h. (AC) RT-qPCR and Western blot analyses were performed to assess the mRNA and protein levels of ABCG5, ABCG8, LXRα, and ACAT2 (n=3/group). Caco-2 cells were pretreated with ACAT2 siRNA. (DF) RT-qPCR and Western blot analyses were performed to evaluate the mRNA and protein levels of ABCG5, ABCG8, LXRα, and ACAT2 in the different groups (n=3/group). The data are expressed as the mean ± SEM, and the results were obtained from three independent experiments. *P <0.05 compared to the control group; #P <0.05 vs the AB23A only group.