Research Paper Volume 12, Issue 24 pp 24604—24622

Control of lifespan and survival by Drosophila NF-κB signaling through neuroendocrine cells and neuroblasts

Figure 6. Inhibition of Toll pathway signaling in IPCs improves survival in stress conditions. (A, C, E, G) Survival, median survival, and 10% max survival of dilp2-Gal4/UAS-Cactus females (blue), dilp5-Gal4/UAS-Cactus females (brown), wor-Gal4/UAS-Cactus females (orange), and UAS-Cactus/+ (black) control females exposed to 5% H2O2 (A), 20mM paraquat (C), starvation (E), or 12μM tunicamycin (G). (B, D, F, H) Survival, median survival, and 10% max survival of dilp2-Gal4/+;UAS-DifRNAi/+ females (blue), dilp5-Gal4/+;UAS-DifRNAi/+ females (brown), wor-Gal4/+;UAS-DifRNAi/+ females (orange), and +/+;UAS-DifRNAi/+ (black) control females exposed to 5% H2O2 (B), 20mM paraquat (D), starvation (F), or 12μM tunicamycin (H). (I, J) 24 hr survival of UAS-Cactus crosses (I) or UAS-DifRNAi crosses (J) outlined above after exposure to 37° C heat stress. (K, L) 24 and 48 hr survival of UAS-Cactus crosses (K) or UAS-DifRNAi crosses (L) outlined above after exposure to 0° C cold stress and allowed to recover for 24 or 48hr. Data information: statistics for curve comparisons are shown in the figure. Error bars represent mean ± SEM. * p<.05, *** p<.001, n.s. not significant (Student’s t-test). n = at least 100 flies for each genotype in stress assays (AH). n = 4-5 vials of 20 flies for each genotype in temperature stress assays (IL).