Research Paper Volume 13, Issue 1 pp 389—410

DOK3 is involved in microglial cell activation in neuropathic pain by interacting with GPR84

Downregulation of DOK3 reduces the levels of inflammatory cytokines in microglia. Microglia were infected with a DOK3-specific short hairpin RNA (shRNA) to establish stably down-regulated DOK3 expression. Total cell lysates of DOK3-shRNA and Scr-shRNA microglia were used to determine the levels of mRNA for DOK3 (A), TNF-α (B), IL-1β (C), IL-6 (D), iNOS (E), P38 (F), CD68 (G), CD11B (H), Arg1 (I), and CD206 (J) by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). N=3 per group, and data are presented as means ± SEM. *p  0.05, #p  0.01 vs. Scr-shRNA.

Figure 1. Downregulation of DOK3 reduces the levels of inflammatory cytokines in microglia. Microglia were infected with a DOK3-specific short hairpin RNA (shRNA) to establish stably down-regulated DOK3 expression. Total cell lysates of DOK3-shRNA and Scr-shRNA microglia were used to determine the levels of mRNA for DOK3 (A), TNF-α (B), IL-1β (C), IL-6 (D), iNOS (E), P38 (F), CD68 (G), CD11B (H), Arg1 (I), and CD206 (J) by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). N=3 per group, and data are presented as means ± SEM. *p < 0.05, #p < 0.01 vs. Scr-shRNA.