Research Paper Volume 13, Issue 1 pp 389—410

DOK3 is involved in microglial cell activation in neuropathic pain by interacting with GPR84

GPR84 proteins are physically associated with DOK3 in microglia. (A) Microglia were pretreated with LPS (1 μM) for 4 or 8 h, and the levels of GPR84 and DOK3 protein were assayed by immunofluorescence analysis. (B) Microglia were pretreated with 6-OAU (1μ M) for 1 or 2 h, and the levels of GPR84 and DOK3 protein were assayed by immunofluorescence analysis. Scale bar, 10 μm. (C) Microglia were pretreated with LPS (1 μM) for 4 h, and the levels of GPR84 in total cell lysates were determined by co-immunoprecipitation and western blotting. (D) Purified recombinant human GST or GST-DOK3 proteins were incubated with microglia for 2 h in reaction buffers. Total cell lysates or reaction products were then used in GST-pulldown assays and western blotting to determine the levels of GPR84.

Figure 3. GPR84 proteins are physically associated with DOK3 in microglia. (A) Microglia were pretreated with LPS (1 μM) for 4 or 8 h, and the levels of GPR84 and DOK3 protein were assayed by immunofluorescence analysis. (B) Microglia were pretreated with 6-OAU (1μ M) for 1 or 2 h, and the levels of GPR84 and DOK3 protein were assayed by immunofluorescence analysis. Scale bar, 10 μm. (C) Microglia were pretreated with LPS (1 μM) for 4 h, and the levels of GPR84 in total cell lysates were determined by co-immunoprecipitation and western blotting. (D) Purified recombinant human GST or GST-DOK3 proteins were incubated with microglia for 2 h in reaction buffers. Total cell lysates or reaction products were then used in GST-pulldown assays and western blotting to determine the levels of GPR84.