Research Paper Volume 13, Issue 1 pp 389—410

DOK3 is involved in microglial cell activation in neuropathic pain by interacting with GPR84

CCI-induced neuropathic pain and inflammatory responses are partially inhibited in DOK3-/- mice. To induce neuropathic pain, B129 and DOK3-/- mice underwent surgery for chronic constriction of the sciatic nerve to establish the CCI model. (A) CCI-induced mechanical allodynia was determined by calculating PWMT on days 0, 3, 7, 14, and 21 after CCI surgery. N=8-10, data are presented as means ± SEM. *p  0.05 vs. sham group, & p B–E) Homogenates of lumbar spinal cord isolated from mice were used to determine the levels of mRNA for DOK3 (B), TNF-α (C), IL-1β (D), and IL-6 (E) on day 7 after CCI. (F–K) Iba-1 (F), P-p38 (G), and GPR84 (H) in lumbar spinal cords of mice were assayed by immunofluorescence and immunohistochemical analysis. Quantities of Iba-1 (I), P-p38 (J), and GPR84 (K) were determined by calculating the integral optical density (IOD). N=8-10, *p p

Figure 4. CCI-induced neuropathic pain and inflammatory responses are partially inhibited in DOK3-/- mice. To induce neuropathic pain, B129 and DOK3-/- mice underwent surgery for chronic constriction of the sciatic nerve to establish the CCI model. (A) CCI-induced mechanical allodynia was determined by calculating PWMT on days 0, 3, 7, 14, and 21 after CCI surgery. N=8-10, data are presented as means ± SEM. *p < 0.05 vs. sham group, & p < 0.05 vs. WT plus CCI at the same time. (BE) Homogenates of lumbar spinal cord isolated from mice were used to determine the levels of mRNA for DOK3 (B), TNF-α (C), IL-1β (D), and IL-6 (E) on day 7 after CCI. (FK) Iba-1 (F), P-p38 (G), and GPR84 (H) in lumbar spinal cords of mice were assayed by immunofluorescence and immunohistochemical analysis. Quantities of Iba-1 (I), P-p38 (J), and GPR84 (K) were determined by calculating the integral optical density (IOD). N=8-10, *p < 0.05; #p < 0.01; NS, not significant when comparing the 2 groups connected by the horizontal line; scale bar, 50 μm.