Figure 5. The effects of HAND2-AS1 on the proliferation, migration and invasion of TNBC cells. (A) HAND2-AS1 or si-HAND2-AS1 was transfected into BT549 cells, and transfection efficiency of HAND2-AS1 or si-HAND2-AS1 was detected using qRT-PCR. n = 6, *p<0.05. (B) MTT assay for BT549 cells. n = 10, *p<0.05. (C) would healing assay was to detected migration of BT549 cells. n = 4, *p<0.05. (D) Transwell assay was to determine invasion of BT549 cells. n = 4, *p<0.05. (E) Clone formation assay was to evaluate proliferation of BT549 cells. n = 4, *p<0.05. The above measurement data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate.