Research Paper Volume 13, Issue 1 pp 437—449

Accumulation of LOX-1+ PMN-MDSCs in nasopharyngeal carcinoma survivors with chronic hepatitis B might permit immune tolerance to epstein–barr virus and relate to tumor recurrence

LOX-1+ PMN-MDSCs suppressed functional T cells in a ROS-dependent manner. (A, B) Effect of ROS inhibitor NAC on LOX-1+ PMN-MDSC function in NPC survivors with CHB. Autologous T cells were stimulated with anti-CD3 and anti-CD28, cocultured with LOX-1+ PMN-MDSCs from NPC survivors with CHB at a 2:1 ratio with NAC or PBS treatment. Evaluation of T cell proliferation by CFSE labeling and IFN-γ production in supernatants by ELISA. Representative flow cytometry data (A), cumulative data, and concentration of IFN-γ in the media (B) are shown (n = 3). (C, D) Effect of ROS inhibitor NAC on LOX-1+ PMN-MDSC function in CHB patients. Representative flow cytometry data (C), cumulative data, and concentration of IFN-γ in the media (D) are shown (n = 3)*, P P

Figure 4. LOX-1+ PMN-MDSCs suppressed functional T cells in a ROS-dependent manner. (A, B) Effect of ROS inhibitor NAC on LOX-1+ PMN-MDSC function in NPC survivors with CHB. Autologous T cells were stimulated with anti-CD3 and anti-CD28, cocultured with LOX-1+ PMN-MDSCs from NPC survivors with CHB at a 2:1 ratio with NAC or PBS treatment. Evaluation of T cell proliferation by CFSE labeling and IFN-γ production in supernatants by ELISA. Representative flow cytometry data (A), cumulative data, and concentration of IFN-γ in the media (B) are shown (n = 3). (C, D) Effect of ROS inhibitor NAC on LOX-1+ PMN-MDSC function in CHB patients. Representative flow cytometry data (C), cumulative data, and concentration of IFN-γ in the media (D) are shown (n = 3)*, P < 0.05; ***, P < 0.001. Abbreviations: PMN-MDSC, polymorphonuclear myeloid-derived suppressor cell; ROS, reactive oxygen species; PMN, polymorphonuclear cell; NPC, nasopharyngeal carcinoma; CHB, chronic hepatitis B; CFSE, 5,6-carboxyfluoresceindiacetate diacetate; IFN, interferon.