Research Paper Volume 13, Issue 1 pp 493—515

Melatonin alleviates angiotensin-II-induced cardiac hypertrophy via activating MICU1 pathway

Knockdown of MICU1 exacerbated Ang-II-induced cardiomyocyte hypertrophy in vitro. (A, B) Western blotting was used to measure the transfection efficiency of MICU1 siRNA in neonatal mice ventricular myocytes (NMVMs) (A) and Ang-II treated NMVMs (B). (C, D) Cell surface areas were measured in neonatal mice ventricular myocytes (NMVMs) stimulated with Ang-II (C) and representative images of α-actinin (red)-and DAPI (blue)-stained cardiomyocytes were followed by cell area quantifications (D). Scale bars=10 μm. (E–G) Western blotting was used to measure protein levels of ANP (E), BNP (F) and β-MHC (G) in NMVMs. (H) The mRNA expression of ANP, BNP and β-MHC in NMVMs was detected by qRT-PCR. ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; β-MHC, β-myosin heavy chain. All the data represent the means ± SEM. N=6-8/group. **P##P&&PΨΨP

Figure 3. Knockdown of MICU1 exacerbated Ang-II-induced cardiomyocyte hypertrophy in vitro. (A, B) Western blotting was used to measure the transfection efficiency of MICU1 siRNA in neonatal mice ventricular myocytes (NMVMs) (A) and Ang-II treated NMVMs (B). (C, D) Cell surface areas were measured in neonatal mice ventricular myocytes (NMVMs) stimulated with Ang-II (C) and representative images of α-actinin (red)-and DAPI (blue)-stained cardiomyocytes were followed by cell area quantifications (D). Scale bars=10 μm. (EG) Western blotting was used to measure protein levels of ANP (E), BNP (F) and β-MHC (G) in NMVMs. (H) The mRNA expression of ANP, BNP and β-MHC in NMVMs was detected by qRT-PCR. ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; β-MHC, β-myosin heavy chain. All the data represent the means ± SEM. N=6-8/group. **P<0.01 vs. Vehicle in Normal; ##P<0.01 vs. Vehicle in Ang-II; &&P<0.01 vs. Normal; ΨΨP<0.01 vs. M scRNA of Ang-II.