Figure 5. Adenoviral overexpression of MICU1 was resistant to cardiomyocyte hypertrophy in vitro. (A, B) Representative immunoblots and quantification of protein levels of MICU1 in (NMVMs) (A) and Ang-II treated NMVMs (B) infected with Ad-EV and Ad-MICU1 were shown. (C, D) Cell surface areas were measured in NMVMs stimulated with Ang-II (C) and representative images of α-actinin (red)-and DAPI (blue)-stained cardiomyocytes (left) were followed by cell area quantifications (D). Scale bars=10 μm. (E–H) Western blotting and qRT-PCR were used to measure protein levels of ANP, BNP and β-MHC in NMVMs and Ang-II treated NMVMs. All the data represent the means ± SEM. N=6-8/group. **P<0.01 vs. Vehicle in Normal; ##P<0.01 vs. Vehicle in Ang-II; &&P<0.01 vs. Normal; ΨΨP<0.01 vs. Ad-EV of Ang-II.