Research Paper Volume 13, Issue 1 pp 493—515

Melatonin alleviates angiotensin-II-induced cardiac hypertrophy via activating MICU1 pathway

Mitochondrial disorder contributed to cardiac hypertrophy induced by MICU1 deficiency. (A, E) Mitochondrial morphology was observed by transmission electron microscopy in control, MICU1 knockdown (A) and MICU1 overexpression mice (E). Scale bars=500 nm. (B, F) Mitochondrial mass was analyzed by Image J in different mice. (C, G) Mitochondrial membrane potential (ΔΨm) level was measured by JC-1 kits in different mice. (D, H) Mitochondrial ATP content was determined by frefly luciferase-based ATP assay kits. All the data represent the means ± SEM. N=6-8/group. **P##P&&PΨΨP

Figure 7. Mitochondrial disorder contributed to cardiac hypertrophy induced by MICU1 deficiency. (A, E) Mitochondrial morphology was observed by transmission electron microscopy in control, MICU1 knockdown (A) and MICU1 overexpression mice (E). Scale bars=500 nm. (B, F) Mitochondrial mass was analyzed by Image J in different mice. (C, G) Mitochondrial membrane potential (ΔΨm) level was measured by JC-1 kits in different mice. (D, H) Mitochondrial ATP content was determined by frefly luciferase-based ATP assay kits. All the data represent the means ± SEM. N=6-8/group. **P<0.01 vs. Control (1); ##P<0.01 vs. M scRNA of Hypertrophy; &&P<0.01 vs. Control (2); ΨΨP<0.01 vs. Ad-EV of Hypertrophy.