Figure 3. Knockdown of EPHA5 in H1299 cells impaired immune related pathways enrichment and expression of immune checkpoint genes. EPHA5 depletion in H1299 cells reduced the enrichment of (A) IFN-γ, (B) TNF, and (C) NF-κB signaling pathway components. (D) EPHA5 KD in H1299 cells downregulated the expression of PDL1 (CD274), IDO1 and CXCL10. **: P < 0.01, ***: P < 0.001.