Research Paper Volume 13, Issue 1 pp 646—674

MITF functions as a tumor suppressor in non-small cell lung cancer beyond the canonically oncogenic role

MITF promotes cell invasion and migration in lung adenocarcinoma cells. (A) The silencing efficiency of shMITF to different MITF isoforms in CL1-0 cells was measured by isoform-specific RT-PCR. Controls included RT-PCR for the common MITF exons 5-9 and GAPDH. (B) The cell invasive ability of CL1-0 cells was assayed after transiently delivering the shMITF expressing plasmids with different doses. Mock: vector transfectant; Scramble: scramble transfectant. *p C) The cell invasive ability of CL1-5 cells was assayed after overexpression of MITF-A with different doses. *p D) The cell invasive and migratory abilities of stably MITF-silenced cells were assayed by using Boyden chamber assays with and without Matrigel, respectively. *pE) Re-expressed MITF-A in stably MITF-silenced cells were assayed for the cell invasion and migration. *p F) The anchorage-independent colony formation ability of stably MITF-silenced cells was assayed. Scale bar, 100 μm. *p

Figure 2. MITF promotes cell invasion and migration in lung adenocarcinoma cells. (A) The silencing efficiency of shMITF to different MITF isoforms in CL1-0 cells was measured by isoform-specific RT-PCR. Controls included RT-PCR for the common MITF exons 5-9 and GAPDH. (B) The cell invasive ability of CL1-0 cells was assayed after transiently delivering the shMITF expressing plasmids with different doses. Mock: vector transfectant; Scramble: scramble transfectant. *p < 0.05 (mean ± SD, n = 3) (C) The cell invasive ability of CL1-5 cells was assayed after overexpression of MITF-A with different doses. *p < 0.05 (mean ± SD, n = 3) (D) The cell invasive and migratory abilities of stably MITF-silenced cells were assayed by using Boyden chamber assays with and without Matrigel, respectively. *p<0.05 (mean ± SD, n = 3) (E) Re-expressed MITF-A in stably MITF-silenced cells were assayed for the cell invasion and migration. *p < 0.05 (mean ± SD, n = 3) (F) The anchorage-independent colony formation ability of stably MITF-silenced cells was assayed. Scale bar, 100 μm. *p < 0.05 (mean ± SD, n = 3).