Research Paper Volume 13, Issue 1 pp 646—674

MITF functions as a tumor suppressor in non-small cell lung cancer beyond the canonically oncogenic role

The regulation landscape and the direct targets of MITF. (A) The volcano plot showed the significant MITF-altered genes with log2-fold-change. Red dot: genes with log2-fold-change ≥ 5, p B) Relative gene expressions in CL1-0 scramble cells and stably MITF-silenced cells assayed by expression microarray and real-time RT-PCR. The scramble and three stably MITF-silenced clones were assayed in triplicate. *p C) MITF binding targets identified by chromatin immunoprecipitation assay. (D) MITF negatively regulated FZD7 expression assayed by immunoblot. (E) The cell invasion of CL1-0 scramble cells and stably MITF-silenced cells assayed after silencing FZD7. *p

Figure 4. The regulation landscape and the direct targets of MITF. (A) The volcano plot showed the significant MITF-altered genes with log2-fold-change. Red dot: genes with log2-fold-change ≥ 5, p < 0.05; Blue dot: genes with log2-fold-change ≤−5, p < 0.05. (B) Relative gene expressions in CL1-0 scramble cells and stably MITF-silenced cells assayed by expression microarray and real-time RT-PCR. The scramble and three stably MITF-silenced clones were assayed in triplicate. *p < 0.05 (mean ± SD, n = 9). (C) MITF binding targets identified by chromatin immunoprecipitation assay. (D) MITF negatively regulated FZD7 expression assayed by immunoblot. (E) The cell invasion of CL1-0 scramble cells and stably MITF-silenced cells assayed after silencing FZD7. *p < 0.05 (mean ± SD, n = 3).