Research Paper Volume 13, Issue 3 pp 3353—3367

Asiatic acid protects oocytes against in vitro aging-induced deterioration and improves subsequent embryonic development in pigs

Changes in the morphology of in vitro aged porcine oocytes after AA supplementation. (A) 24-h in vitro aged oocytes; (B) 24-h in vitro aged oocytes supplemented with 10 μM AA; (C) 48-h in vitro aged oocytes; (D) 48-h in vitro aged oocytes supplemented with 10 μM AA; (E) morphological changes in porcine oocytes recovered at different culture time points from the AA supplementation groups. Representative morphologically abnormal in vitro aged oocytes (white arrows) were examined by optical microscopy. Scale bar=100 μm. The numbers of oocytes examined in each group are shown by the bars. Statistically significant differences are indicated by different letters (p).

Figure 1. Changes in the morphology of in vitro aged porcine oocytes after AA supplementation. (A) 24-h in vitro aged oocytes; (B) 24-h in vitro aged oocytes supplemented with 10 μM AA; (C) 48-h in vitro aged oocytes; (D) 48-h in vitro aged oocytes supplemented with 10 μM AA; (E) morphological changes in porcine oocytes recovered at different culture time points from the AA supplementation groups. Representative morphologically abnormal in vitro aged oocytes (white arrows) were examined by optical microscopy. Scale bar=100 μm. The numbers of oocytes examined in each group are shown by the bars. Statistically significant differences are indicated by different letters (p<0.05).