Research Paper Volume 13, Issue 1 pp 865—876

Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis

Propofol suppresses the rI/R-induced downregulation of SIRT1 in rat alveolar macrophages. NR8383 cells were incubated with serum from sham or rI/R rats, with or without propofol (50 or 100 μM) for 24 h. (A) Real-time PCR was used to measure SIRT1 mRNA levels. (B) Western blotting was used to measure SIRT1 protein levels (*P #P

Figure 2. Propofol suppresses the rI/R-induced downregulation of SIRT1 in rat alveolar macrophages. NR8383 cells were incubated with serum from sham or rI/R rats, with or without propofol (50 or 100 μM) for 24 h. (A) Real-time PCR was used to measure SIRT1 mRNA levels. (B) Western blotting was used to measure SIRT1 protein levels (*P < 0.05 vs. sham; #P < 0.05 vs. rI/R).