Research Paper Volume 13, Issue 1 pp 865—876

Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis

The SIRT1 inhibitor nicotinamide prevents propofol from reducing rI/R-induced pyroptosis in rat alveolar macrophages. NR8383 cells were incubated with serum from sham or rI/R rats, with or without propofol (100 μM) and/or nicotinamide (1 mM) for 24 h. (A) Western blotting was used to measure the protein levels of cleaved caspase 1 (P10), ASC and NLRP3. (B) ELISAs were used to determine the protein levels of IL-18 and IL-1β (*P #P $P

Figure 3. The SIRT1 inhibitor nicotinamide prevents propofol from reducing rI/R-induced pyroptosis in rat alveolar macrophages. NR8383 cells were incubated with serum from sham or rI/R rats, with or without propofol (100 μM) and/or nicotinamide (1 mM) for 24 h. (A) Western blotting was used to measure the protein levels of cleaved caspase 1 (P10), ASC and NLRP3. (B) ELISAs were used to determine the protein levels of IL-18 and IL-1β (*P < 0.05 vs. sham; #P < 0.05 vs. rI/R; $P < 0.05 vs. rI/R + propofol).